Hydration of beta-Lactoglobulin in Magnetized Water: Effect of Magnetic Treatment on the Cluster Structure of Water and Hydration Properties of Proteins

作者：He Jin Song^*; Yang Hong Wei; Cai Ran; Lo Chih Kang; Zhu Wan Peng

来源：Acta Physico - Chimica Sinica, 2010, 26(2): 304-310.

• Magnetization
• Hydrogen bond
• Water molecule cluster
• Protein hydration

To investigate the hydration of proteins in magnetized water, pure water was treated with a static magnetic field (MF) over different effective treatment time (t(eff)). Viscosimetry and oxygen nuclear magnetic resonance ((17)O-NMR) spectra were recorded. The hydration properties of beta-lactoglobulin (beta-Lg) in magnetized water were examined by differential scanning calorimetry (DSC) and NMR. By increasing t(eff), the internal energy of the water decreased and the concentration of "hydrogen-bonded" water increased. This result indicates that MF treatment promotes the formation of water clusters, which can be attributed to an increase in hydrogen bonding. For the beta-Lg solution prepared in magnetized water, the mobility of water molecules around the beta-Lg surface did not change significantly with an increase in t(eff) but the quantity of non-freezable bound water increased. This suggests that beta-Lg hydration is related to the formation of water clusters, which depends on the hydrogen-bonding structure of water and can be varied by MF treatment.

• 出版日期2010-2
• 单位清华大学