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摘要
Purpose: To investigate the protective effect of Suaeda asparagoides (Chenopodiaceae) extract on neuroinflammatory responses induced by lipopolysaccharide (LPS) in BV-2 microglial cells and its antioxidant effects. %26lt;br%26gt;Methods: Biochemical studies carried out include 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) assay and 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) assay for cell viability and radical scavenging activities, respectively. To evaluate the anti-neuroinflammatory effects of S. asparagoides (SAE) extract, LPS (1 mu g/ml)-stimulated BV-2 microglial cells were used and pro-inflammatory mediators and cytokines such as nitric oxide (NO), inducible NO (iNOS), cyclooxygenase (COX)-2, tumor necrosis factor-alpha (TNF-alpha) and nuclear factor-kappa B (NF-kappa B) were measured using Western blotting and enzyme-linked immunosorbent assay (ELISA). %26lt;br%26gt;Results: LPS-stimulation of BV-2 cells increased the levels of NO (25.2 +/- 2.15, p %26lt; 0.001) and proinflammatory mediators such as iNOS, COX-2 and TNF-alpha. However, treatment with SAE extract (20, 40 and 80 mu g/ml) to LPS-stimulated BV-2 cells significantly inhibited the excessive release of NO (p %26lt; 0.05 at 20 mu g/ml and p %26lt; 0.001 at 40 and 80 mu g/ml, respectively) and suppressed the increased levels of iNOS, COX-2 and TNF-alpha. SAE also concentration dependently inhibited the NF-kB activation in LPSstimulated BV-2 microglia. Further, SAE significantly and concentration-dependently (p %26lt; 0.001 at 20 200 mu g/ml, respectively) scavenged DPPH radicals with IC50 of 36.33 +/- 2.12 mu g/ml. %26lt;br%26gt;Conclusion: The results strongly suggest that SAE exhibits protective activity against LPS-stimulated neuroinflammatory responses. Mechanistic study reveals that SAE might by regulating NF-kappa B signaling. The antioxidant activity exhibited by SAE extract might also play a role in the plant%26apos;s significant antineuroinflammatory effect.
- 出版日期2014-9
