A study of miRNAs targets prediction and experimental validation

作者：Huang Yong; Zou Quan; Song Haitai; Song Fei; Wang Ligang; Zhang Guozheng; Shen Xingjia^*

来源：Protein & Cell, 2010, 1(11): 979-986.

DOI：10.1007/s13238-010-0129-4

• microRNA
• computational prediction
• target
• experimental validation

microRNAs (miRNAs) are 20-24 nucleotide (nt) RNAs that regulate eukaryotic gene expression post-transcriptionally by the degradation or translational inhibition of their target messenger RNAs (mRNAs). To identify miRNA target genes will help a lot by understanding their biological functions. Sophisticated computational approaches for miRNA target prediction, and effective biological techniques for validating these targets now play a central role in elucidating their functions. Owing to the imperfect complementarity of animal miRNAs with their targets, it is difficult to judge the accuracy of the prediction. Complexity of regulation by miRNA-mediated targets at protein and mRNAs levels has made it more challenging to identify the targets. To date, only a few miRNAs targets are confirmed. In this article, we review the methods of miRNA target prediction and the experimental validation for their corresponding mRNA targets in animals.

• 出版日期2010-11
• 单位厦门大学; 中国农业科学院