Urinary clusterin as a renal marker in dogs

作者：Garcia Martinez Juan D^*; Tvarijonaviciute Asta; Ceron Jose J; Caldin Marco; Martinez Subiela Silvia

来源：Journal of Veterinary Diagnostic Investigation, 2012, 24(2): 301-306.

DOI：10.1177/1040638711435112

• Analytical validation
• chronic kidney disease
• clusterin
• dogs
• renal markers
• urine

A validation of a species-specific enzyme immunoassay for urinary clusterin measurement in dogs was performed, and the use of urinary clusterin as a marker of renal damage was evaluated in a population of dogs with leishmaniasis. Urine was obtained from 75 dogs; 64 dogs had leishmaniasis and 11 were healthy. The dogs with leishmanias were divided into 5 groups: I (n = 9; serum creatinine [SCr] %26lt; 1.4 mg/dl, urinary protein-to-creatinine [UPC] ratio %26lt;= 0.5); II (n = 29; SCr %26lt; 1.4 mg/dl, UPC %26gt; 0.5); III (n = 6; SCr %26gt;= 1.4 mg/dl to %26lt; 2 mg/dl, UPC %26gt; 0.5); IV (n = 13; SCr %26gt;= 2 mg/dl to %26lt; 5 mg/dl, UPC %26gt; 0.5); and V (n = 7; SCr %26gt;= 5 mg/dl, UPC %26gt; 0.5). The urinary clusterin concentration was measured, and the urinary clusterin-to-creatinine ratio was calculated. Canine urinary clusterin assay showed good analytical performance based on precision accuracy and limit-of-detection results. There was a statistically significant increase in urinary clusterin and clusterin-to-creatinine ratio in groups II-V compared with group I and healthy group. The results of the current study showed that urinary clusterin concentration and urinary clusterin-to-creatinine ratios are increased in dogs with analytical evidences of renal damage and that the urinary clusterin-to-creatinine ratio might be used as a potential early biomarker of chronic kidney disease.

• 出版日期2012-3