Differential expression alpha B-crystallin causes maturation-dependent susceptibility of oligodendrocytes to oxidative stress

作者：Kim Ji Young; Lee Eun Young; Sohn Hyun Joon; Kim Si Wook; Kim Chan Hyung; Ahn Hee Yul; Kim Dong Woon; Cho Sa Sun; Seo Je Hoon^*

来源：BMB Reports, 2013, 46(10): 501-506.

DOI：10.5483/BMBRep.2013.46.10.015

• alpha B-crystallin
• Cell survival
• Oligodendrocyte
• Oligodendrocyte precursor cell
• Oxidative stress

Oligodendrocyte precursor cells (OPCs) are most susceptible to oxidative stress in the brain. However, the cause of differences in susceptibility to oxidative stress between OPCs and mature oligodendrocytes (mOLs) remains unclear. Recently, we identified in vivo that alpha B-crystallin (aBC) is expressed in mOLs but not in OPCs. Therefore, we examined in the present study whether aBC expression could affect cell survival under oxidative stress induced by hydrogen peroxide using primary cultures of OPCs and mOLs from neonatal rat brains. Expression of aBC was greater in mOLs than in OPCs, and the survival rate of mOLs was significantly higher than that of OPCs under oxidative stress. Suppression of aBC by siRNA transfection resulted in a decrease in the survival rate of mOLs under oxidative stress. These data suggest that higher susceptibility of OPCs than mOLs to oxidative stress is due, at least in part, to low levels of aBC expression.

• 出版日期2013-10-31