A resource dependent protein synthesis model for evaluating synthetic circuits

作者：Halter Wolfgang^*; Montenbruck Jan Maximilian; Tuza Zoltan A; Allgoewer Frank

来源：Journal of Theoretical Biology, 2017, 420: 267-278.

DOI：10.1016/j.jtbi.2017.03.004

• Synthetic biology
• Host-circuit interactions
• Resource dependence
• Genetic regulatory networks
• Normally hyperbolic manifolds

Reliable in silico design of synthetic gene networks necessitates novel approaches to model the process of protein synthesis under the influence of limited resources. We present such a novel protein synthesis model which originates from the Ribosome Flow Model and among other things describes the movement of RNA-polymerase and ribosomes on mRNA and DNA templates, respectively. By analyzing the convergence properties of this model based upon geometric considerations, we present additional insights into the dynamic mechanisms of the process of protein synthesis. Further, we demonstrate how this model can be used to evaluate the performance of synthetic gene circuits under different loading scenarios.

• 出版日期2017-5-7