alpha-Amylase Production by Bacillus licheniformis under Solid State Fermentation Conditions and its Cross Linking with Metalosalts to Confer Thermostability

作者：Niaz Mubashir^*; Iftikhar Tehreema; Tabassum Romana; Zia Muhammad Anjum; Saleem Humaira; Abbas Syed Qaiser; Haq Ikram U

来源：International Journal of Agriculture and Biology, 2010, 12(5): 793-795.

• alpha-Amylase
• Solid state fermentation
• Bacillus licheniformis
• Stability

The production potential of Bacillus licheniformis strain RT(7)YC for extracellular alpha-amylase was analyzed through solid state fermentation. Enzyme concentration produced under varying concentrations of substrate and ecological variables was calculated by using DNS and starch-iodine methods. The maximum production of alpha-amylase was obtained at 20% wheat bran. It was observed that corn steep liquor was found as best nitrogen source for alpha-amylase production. It was also found that enzyme was stable at 60 degrees C when treated for 10 min. Moreover, it was also much stable at 10 mM concentration of CaCl(2).

• 出版日期2010-9