科研之友
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摘要
Herein, an autonomous table-tennis-motion-type DNA machine (ATT DNA machine) was proposed to integrate target recycling amplification (TRA) and triple strand-displacement amplification (T-SDA) into one signaling system for the efficient amplification detection of p53 gene. Firstly, X-shaped DNA duplex probe (XSP) was formed via the simple annealing of Probe A and Probe B. Upon addition of p53, the hybridization of p53 with Probe A results in the peeling of Probe B from XSP. Then, the primer binds to the Probe B to trigger a circular strand-displacement amplification, during which new primers are generated. Moreover, the newly-produced primers can in turn activate the target recycling amplification and triple strand-displacement amplification via bouncing back and forth between different Probe As and Probe Bs in a manner analogous to the table tennis motion. This newly-developed DNA machine can be used to detect p53 down to 0.5 pM with an excellent specificity, offering a 100-fold enhanced sensitivity compared to a non-amplification one-to-one hybridization based system. Moreover, this DNA machine is able to be applied for target analysis in complex environments. Additionally, the potential for practical applications is also verified, demonstrating a promising alternative tool for early diagnosis of cancers.
