Superior In Vitro Stimulation of Human CD8(+) T-Cells by Whole Virus versus Split Virus Influenza Vaccines

作者：Halbroth Benedict R; Heil Alexander; Distler Eva; Dass Martin; Wagner Eva M; Plachter Bodo; Probst Hans Christian; Strand Dennis; Hartwig Udo F; Karner Anita; Aichinger Gerald; Kistner Otfried; Landfester Katharina; Herr Wolfgang^*

来源：PLos One, 2014, 9(7): e103392.

DOI：10.1371/journal.pone.0103392

Pandemic and seasonal influenza viruses cause considerable morbidity and mortality in the general human population. Protection from severe disease may result from vaccines that activate antigen-presenting DC for effective stimulation of influenza-specific memory T cells. Special attention is paid to vaccine-induced CD8(+) T-cell responses, because they are mainly directed against conserved internal influenza proteins thereby presumably mediating cross-protection against circulating seasonal as well as emerging pandemic virus strains. Our study showed that influenza whole virus vaccines of major seasonal A and B strains activated DC more efficiently than those of pandemic swine-origin H1N1 and pandemic-like avian H5N1 strains. In contrast, influenza split virus vaccines had a low ability to activate DC, regardless which strain was investigated. We also observed that whole virus vaccines stimulated virus-specific CD8(+) memory T cells much stronger compared to split virus counterparts, whereas both vaccine formats activated CD4(+) Th cell responses similarly. Moreover, our data showed that whole virus vaccine material is delivered into the cytosolic pathway of DC for effective activation of virus-specific CD8(+) T cells. We conclude that vaccines against seasonal and pandemic (-like) influenza strains that aim to stimulate cross-reacting CD8(+) T cells should include whole virus rather than split virus formulations.

• 出版日期2014-7-29