AUTOMATED DETERMINATION OF HYDROGEN PEROXIDE AT THE MICRO-MOLAR LEVEL IN RAINWATER AND SNOW USING A STOPPED-FLOW APPROACH IN A HYBRID SEQUENTIAL INJECTION/FLOW INJECTION MANIFOLD

作者：Tzanavaras Paraskevas D^*; Boulimari Euridiki

来源：Analytical Letters, 2012, 45(9): 1086-1097.

DOI：10.1080/00032719.2012.670798

• Ellman%26apos
• s reagent
• Hybrid sequential injection/flow injection
• Hydrogen peroxide
• Stopped-flow
• Tris(2-carboxyethyl) phosphine

A new automated method is reported for the determination of H2O2 in real samples. The method is based on the quenching effect of the analyte on the reaction between tris(2-carboxyethyl) phosphine (TCEP) and Ellman%26apos;s reagent (DTNB). All necessary steps were accomplished under flow conditions using a hybrid sequential injection (SI)/flow injection (FI) setup. The sensitivity was enhanced by applying a stopped-flow step (120s) in order to promote the reaction between H2O2 and TCEP. The proposed analytical protocol was validated for linearity (10-75 mu mol L-1), limits of detection (c(L) = 1.0 mu mol L-1), quantitation (c(Q) = 3.3 mu mol L-1), precision (s(r) = 1.3-1.7%), accuracy, and selectivity. It was then applied successfully to the analysis of H2O2 in spiked rainwater and snow samples.

• 出版日期2012