Adsorption of peroxidase from Raphanus sativus L onto alginate-guar gum matrix: Kinetic, equilibrium and thermodynamic analysis

作者：Santos Leite da Silva Ana C; Woitovich Valetti Nadia; Brassesco Maria E; Teixeira Jose A; Pico Guillermo^*

来源：Adsorption Science and Technology, 2016, 34(6): 388-402.

DOI：10.1177/0263617416659287

• Alginate
• guar gum
• polyelectrolytes
• peroxidase
• adsorption
• isotherms

This work explores the kinetics, equilibrium and thermodynamics of peroxidase adsorption onto spherical guar gum-alginate matrices. The effect of contact time, solution pH, initial protein concentration and temperature was studied in batch experiments. The results show that peroxidase adsorption increased with rising contact time and initial enzyme concentration, and was higher at pH 4.0. The kinetic processes can be predicted by both the pseudo-first-order rate kinetics and the pseudo-second-order rate kinetics. Equilibrium adsorption data were analyzed with different isotherm models. The experimental data fitted to the Freundlich model in agreement with the low energy activation, demonstrating the presence of a high physical and unspecific interaction between the enzyme and the matrix.

• 出版日期2016-7