Antibiotic resistance of foodborne multidrug-resistant listeria monocytogenes

作者：Li, Li-Li; Shi, Lei; He, Jian-Hua; Yan, He; Meng, He-Cheng

来源：Modern Food Science and Technology, 2015, 31(7): 105-110.

DOI：10.13982/j.mfst.1673-9078.2015.7.018

• Antibiotic resistance
• Food-borne pathogens
• Listeria monocytogenes
• Minimum inhibitory concentration
• Multidrug resistance
• Multidrug resistants
• Natural transformations
• Plasmid

The mechanism of antibiotic resistance in foodborne, multidrug-resistant (MDR) Listeria monocytogenes was investigated to develop measures to control the MDR phenomenon. Genetic factors of antibiotic-resistance were screened by polymerase chain reaction (PCR), the position and dissemination capacity of these factors were evaluated by plasmid curing and natural transformation, and the stability of drug-resistance dissemination was verified by consecutive subculture. The results showed that the MDR strain LM78 (resistant to chloramphenicol, erythromycin, streptomycin, tetracycline, trimethoprim/sulfamethoxazole) carried the cat, ermB, and tetS genes. Following plasmid curing, the minimum inhibitory concentration (MIC) was reduced to the sensitive range. Moreover, the plasmid could be transferred between species by natural transformation. Plasmid curing and transformation results suggested that these resistance genes were encoded in the plasmid. After consecutive subculture without antibiotics, these plasmids remained sufficiently stable. The MDR trait of foodborne pathogens may be transferred between bacterial species, and subsequent infection in humans via contaminated foodposes a potential threat against human health.

• 出版日期2015-7-15
• 单位华南理工大学