Arsenic in an As-contaminated Abandoned Mine was Mobilized from Fern-rhizobium to Frond-bacteria via the ars Gene

作者：Chang Jin Soo^*; Lee Se Yong; Kim Kyoung Woong

来源：Biotechnology and Bioprocess Engineering, 2010, 15(5): 862-873.

DOI：10.1007/s12257-009-3154-5

• arsenic-contaminated abandoned mine
• arsenic resistance system (ars)
• ars genotype
• fern-rhizosphere/rhizoplane
• fern-rhizobium
• frond-bacteria
• stipe-bacteria

The mobilization of arsenic from fern-rhizobium to frond-bacteria in As-contaminated abandoned mine environments was found to be induced by the ars gene. Uncultured Acinetobacter sp. strain MRI67 (DQ539027) and uncultured Buttiaxella sp. strain MRI-65 (DQ539024) were identified in the root/rhizosphere based on the presence of the ars gene. The arsenite-oxidizing bacterial strains isolated in this study were found to grow in the presence of 14 mM sodium arsenite (NaAsO(2)). In addition, the concentration of arsenic in five grams of Deparia lobatocrenata As-8 (EU476190) obtained from the Myoungbong abandoned mine area was 8,900 mg/kg +/- 56.9, whereas the arsenic concentrations in the frond-rhizoplane of Pseudomonas putida GIST-MRP44-1 (EF623836), the root-rhizoplane of Acinetobacter sp. GIST-MRO62 (EF623849), and the stipe-rhizoplane of P putida GIST-MRO63 (EF623850) were 41.9 mg/kg +/- 1.0 (1 g of tissue), 32.9 mg/kg +/- 0.9 (1 g of tissue), and 17.9 mg/kg +/- 1.4 (1 g of tissue), respectively. Taken together, these findings suggest that the newly isolated indigenous fern-rhizobium, stipe-bacteria, and frond-bacteria may provide a better understanding of arsenic mobility in the field of molecular geomicrobiology and that it can be applied to the phytoremediation of arsenic-contaminated mines.

• 出版日期2010-10