A fast and accurate method for the pharmacokinetic research of four coumarin analogs in Fructus cnidii using capillary electro-chromatography with a methacrylate ester-based monolithic column

作者：Chen, Zhao; Xu, Aili; Bi, Xiaoli; Luo, Wenhui; Li, Ji; Fan, Guorong^*; Sun, Dongmei

来源：Electrophoresis, 2017, 38(22-23): 3036-3047.

DOI：10.1002/elps.201770184

• Capillary electro-chromatography
• Methacrylate ester-based monolithic column
• Fructus cnidii
• Coumarin analogs
• Pharmacokinetic research

In the present study, a monolithic capillary column with higher permeability was developed for the in vivo discrimination of four coumarin analogs (bergapten, 2-acetylangelicin, imperatorin, and osthole) that typically require long separation times in HPLC. Instead of conventional methacrylate ester monolith (containing 19.5% porogen) with insufficient permeability (K=1.52 - 1.66x10(-14) M-2) for plasma sample, the proposed column (20.5% porogen) had better permeability (around 3.80x10(-14) M-2) while properties such as pore distribution, stability, and resolution changed slightly. As a result, due to the negatively charged electro-dynamic flow of the methacrylate ester groups in the monolith, the migration of targeted analytes was achieved within 6min (compared with 30min in HPLC) with acceptable resolution and improved sensitivity (0.005-0.02g/mL vs. 0.04g/mL). The proposed method was also applied to pharmacokinetic research: accelerated solvent extraction (ASE) was used to improve the extraction efficiency, which prepared extract much faster and more pure than conventional methods. As the pharmacokinetic parameters indicated, the monolithic capillary electro-chromatography method was efficient, sensitive, specific, and durable, guaranteeing its utility for the determination of multiple structure-related compounds in rat plasma.

• 出版日期2017-11
• 单位昆明医科大学; 广州中医药大学; 中国人民解放军第二军医大学