A microelectrode biosensor for real time monitoring of L-glutamate release

作者：Tian Faming^*; Gourine Alexander V; Huckstepp Robert T R; Dale Nicholas

来源：Analytica Chimica Acta, 2009, 645(1-2): 86-91.

DOI：10.1016/j.aca.2009.04.048

• L-Glutamate
• Sol-gel
• Microbiosensor
• In vivo
• In vitro

We have developed an amperometric microbiosensor for real time monitoring L-glutamate release in neural tissue, based on enzymatic oxidation catalyzed by the L-glutamate oxidase. By means of a sol-gel coating method, L-glutamate oxidase was entrapped in a biocompatible gel layer that provided a benign environment and retained enzyme activity on the surface of Pt microelectrode. Prior to gel layer formation, a modification on the surface of Pt microelectrode with poly(phenylene diamine) enabled the microbiosensor screen majority of common potential interfering substances existing in physiological samples. The miniaturized biosensor achieved a steady state response to L-glutamate within 10s and exhibited a linear dependence on the concentration of L-glutamate from 0.5 to 100 mu mol L(-1) with a high sensitivity of 279.4 +/- 2.0 mu A(mmol L(-1))(-1) cm(-2) (n=4, R.S.D. = 2.8%). The microbiosensor also exhibited excellent long-term stability in dry storage. We have successfully used the microbiosensor for real time measuring of L-glutamate in vivo.

• 出版日期2009-7-10