科研之友
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摘要
BackgroundNebulized surfactant therapy has been proposed as an alternative method of surfactant administration. The use of a perforated vibrating membrane nebulizer provides a variety of advantages over conventional nebulizers. We investigated the molecular structure and integrity of poractant alfa pre- and post-nebulization. %26lt;br%26gt;MethodCurosurf (R) was nebulized using an Investigational eFlow (R) Nebulizer System. Non-nebulized surfactant (NN), recollected surfactant droplets from nebulization through an endotracheal tube (NT) and nebulization of surfactant directly onto a surface (ND) were investigated by transmission electron microscopy. Biophysical characteristics were assessed by the Langmuir-Wilhelmy balance and the Captive Bubble Surfactometer. %26lt;br%26gt;ResultsVolume densities of lamellar body-like forms (LBL) and multi-lamellar forms (ML) were high for NN and NT samples (38.8% vs. 47.7% for LBL and 58.2% vs. 47.8% for ML). In the ND sample, we found virtually no LBL%26apos;s, ML%26apos;s (72.6%) as well as uni-lamellar forms (16.4%) and a new structure, the garland-like forms (9.4%). Surface tension for NN and NT was 23.330.29 and 25.77 +/- 1.12mN/m, respectively. Dynamic compression-expansion cycling minimum surface tensions were between 0.91 and 1.77mN/m. %26lt;br%26gt;ConclusionThe similarity of surfactant characteristics of nebulized surfactant via a tube and the non-nebulized surfactant suggests that vibrating membrane nebulizers are suitable for surfactant nebulization. Alterations in surfactant morphology and characteristics after nebulization were transient. A new structural subtype of surfactant was identified. Pediatr Pulmonol. 2014; 49:348-356.
- 出版日期2014-4
