ERK2-ZEB1-miR-101-1 axis contributes to epithelial-mesenchymal transition and cell migration in cancer

作者：Mangalhara Kailash Chandra; Manvati Siddharth; Saini Sunil Kumar; Ponnusamy Kalaiarasan; Agarwal Gaurav; Abraham Suresh K; Bamezai Rameshwar N K^*

来源：Cancer Letters, 2017, 391: 59-73.

DOI：10.1016/j.canlet.2017.01.016

• microRNA
• miR-101
• Breast cancer
• EMT-TFs
• Cytoskeleton
• Epidermal growth factor (EGF)

Regulation of metastasis continues to remain enigmatic despite our improved understanding of cancer. Identification of microRNAs associated with metastasis in the recent past has provided a new hope. Here, we show how microRNA-101 (miR-101) regulates two independent processes of cellular metastasis by targeting pro-metastatic upstream regulatory transcription factors, ZEB1 and ZEB2, and downstream effector-actin modulators, RHOA and RAC1, providing a single target for therapeutic intervention. Further, we depict how down-regulation of miR-101 by extracellular signal-regulated kinase-2 (ERK2) is vital for MAP kinase pathway induced cellular migration and mesenchymal transition. Importantly, EKR2 induced expression of ZEB1 seems essential for down-regulation of miR-101-1 and induction of EMT. Given the role of EMT in metastasis, we also observe a significant correlation between miR-101 expression and lymph node metastasis; and identify the ERK2-ZEB1-miR-101-1 pathway active in breast cancer tissues, with an apparent clinicopathological implication.

• 出版日期2017-4-10
• 单位河北医科大学