Ultrafast, large-field multiphoton microscopy based on an acousto-optic deflector and a spatial light modulator

作者：Shao, Yonghong; Qin, Wan; Liu, Honghai; Qu, Junle; Peng, Xiang; Niu, Hanben; Gao, Bruce Z^*

来源：Optics Letters, 2012, 37(13): 2532-2534.

DOI：10.1364/OL.37.002532

• LASER-SCANNING MICROSCOPY
• TEMPORAL DISPERSION
• COMPENSATION

We present an ultrafast, large-field multiphoton excitation fluorescence microscope with high lateral and axial resolutions based on a two-dimensional (2-D) acousto-optical deflector (AOD) scanner and spatial light modulator (SLM). When a phase-only SLM is used to shape the near-infrared light from a mode-locked titanium: sapphire laser into a multifocus array including the 0-order beam, a 136 mu m x 136 mu m field of view is achieved with a 60x objective using a 2-D AOD scanner without any mechanical scan element. The two-photon fluorescence image of a neuronal network that was obtained using this system demonstrates that our microscopy permits observation of dynamic biological events in a large field with high-temporal and -spatial resolution.

• 出版日期2012-7-1
• 单位深圳大学