Altered muscle satellite cell activation following 16 wk of resistance training in young men

作者：Nederveen Joshua P; Snijders Tim; Joanisse Sophie; Wavell Christopher G; Mitchell Cameron J; Johnston Leeann M; Baker Steven K; Phillips Stuart M; Parise Gianni^*

来源：American Journal of Physiology - Regulatory, Integrative and Comparative Physiology, 2017, 312(1): R85-R92.

DOI：10.1152/ajpregu.00221.2016

• muscle stem cells
• Pax7
• MyoD
• capillaries
• perfusion

Skeletal muscle satellite cells (SC) play an important role in muscle adaptation. In untrained individuals, SC content and activation status have been observed to increase in response to a single bout of exercise. Muscle fiber characteristics change considerably when resistance exercise is performed chronically, but whether training status affects the activity of SC in response to a single bout of exercise remains unknown. We examined the changes in SC content and activation status following a single bout of resistance exercise, before and following a 16-wk progressive resistance training (RT) program in 14 young (25 +/- 3 yr) men. Before and after RT, percutaneous biopsies from the vastus lateralis muscle were taken before a single bout of resistance exercise and after 24 and 72 h of postexercise recovery. Muscle fiber size, capillarization, and SC response were determined by immunohistochemistry. Following RT, there was a greater activation of SC after 24 h in response to a single bout of resistance exercise (Pre, 1.4 +/- 0.3; 24 h, 3.1 +/- 0.3 Pax7(+) / MyoD(+) cells per 100 fibers) compared with before RT (Pre, 1.4 +/- 0.3; 24 h, 2.2 +/- 0.3 Pax7(+) /MyoD(+) cells per 100 fibers, P < 0.05); no difference was observed 72 h postexercise. Following 16 wk of RT, MyoD mRNA expression increased from basal to 24 h after the single bout of exercise (P < 0.05); this change was not observed before training. Individual capillary-to-fiber ratio (C/Fi) increased in both type I (1.8 +/- 0.3 to 2.0 +/- 0.3 C/Fi, P < 0.05) and type II (1.7 +/- 0.3 to 2.2 +/- 0.3 C/Fi, P < 0.05) fibers in response to RT. After RT, enhanced activation of SC in response to resistance exercise is accompanied by increases in muscle fiber capillarization.

• 出版日期2017-1