Allosyndetic recombinants of the Aegilops peregrina-derived Lr59 translocation in common wheat

作者：Marais G F^*; Kotze L; Eksteen A

来源：Plant Breeding, 2010, 129(4): 356-361.

DOI：10.1111/j.1439-0523.2009.01713.x

• chromosome engineering
• gene transfer
• leaf rust resistance

P>The wild relatives constitute a valuable source of rust resistance genes that can be utilized in wheat breeding. However, translocation of desirable genes through chromosome engineering inevitably results in co-transfer of deleterious wild species chromatin. An attempt was made to replace such redundant alien chromatin on the Lr59 translocation through homoeologous chromosome pairing and crossing over in the absence of Ph1. Strong segregation distortion resulted in the recovery of an unexpectedly high frequency of resistant recombinants. Eight of these retained comparatively short, distal segments of foreign chromatin, including Lr59. The foreign chromatin that remained in the latter plants was characterized with the use of twelve anonymous AFLP loci, the data of which suggested reduced homoeology with 1AL that could have been the result of a sub-terminal, paracentric inversion. Crossing over within an inversion loop may have resulted in a low frequency of genetically unbalanced gametes. It will therefore be necessary to develop near-isogenic lines of the eight recombinants and to do field evaluations in order to identify those that retained the shortest, balanced translocations.

• 出版日期2010-8