Development of electrochemical genosensor for MYCN oncogene detection using rhodamine B as electroactive label

作者：Souza e Silva Thalles Douglas; Honorato de Castro Ana Cristina; Rodovalho Vinicius de Rezende; Madurro Joao Marcos; Brito Madurro Ana Graci

来源：Journal of Solid State Electrochemistry, 2016, 20(9): 2411-2418.

DOI：10.1007/s10008-016-3326-0

• MYCN
• Neuroblastoma
• Rhodamine B
• Genosensor
• Poly(4-aminophenol)

A novel electrochemical genosensor based on a graphite electrode modified with poly(4-aminophenol) has been constructed for prognostic of neuroblastoma, a malignant tumor originating from embryonic precursor cells of the sympathetic nervous system and associated with the amplification of the MYCN oncogene. The genosensor exhibited distinct electrical and morphological properties using rhodamine B as indicator of DNA hybridization. The detection limit was evaluated to be 0.47 mu mol L-1 (n = 3), and the electrochemical genosensor was selective for the complementary DNA, using serum sample. This DNA sensing platform was successfully applied to detect MYCN, an important biomarker for neuroblastoma.

• 出版日期2016-9