A Novel Way to Go Whole Cell in Patch-Clamp Experiments

作者：Inayat Samsoon; Zhao Yan; Cantrell Donald R; Dikin Dmitriy A; Pinto Lawrence H; Troy John B^*

来源：IEEE Transactions on Biomedical Engineering, 2010, 57(11): 2764-2770.

DOI：10.1109/TBME.2010.2055055

• Electrophysiology
• gigaseal
• patch clamp
• whole cell

With a conventional patch-clamp electrode, an Ag/AgCl wire sits stationary inside the pipette. To move from the gigaseal cell-attached configuration to whole-cell recording, suction is applied inside the pipette. We have designed and developed a novel "Pushpen patch-clamp electrode," in which a W wire insulated and wound with Ag/AgCl wire can move linearly inside the pipette. The W wire has a conical tip, which can protrude from the pipette tip like a push pen, a procedure we call the "Pushpen Operation." We use the Pushpen operation to impale the cell membrane in cell-attached configuration to go whole cell without disruption of the gigaseal. We successfully recorded whole-cell currents from Chinese hamster ovarian cells expressing influenza A virus protein A/M2, after obtaining whole-cell configuration with the Pushpen operation. This novel method of achieving whole-cell configuration may have a higher success rate than the conventional patch-clamp technique.

• 出版日期2010-11
• 单位西北大学