Mitochondrial DNA 4401A > G and 4435A > G Mutations Associated with Essential Hypertension Testing Method

作者：Xue Ling; Yu Han; Geng Junwei; Tang Xiaowen^*

来源：Clinical Laboratory, 2015, 61(5-6): 623-629.

DOI：10.7754/Clin.Lab.2015.150111

• essential hypertension
• mitochondrial DNA
• mutation
• nested PCR
• enzyme digestion

Background: Establish a method to identify simultaneously mitochondrial DNA 4401A>G and 4435A>G mutations associated with essential hypertension.
Methods: The whole genomic DNA of samples carrying mitochondria! DNA 4401A>G and 4435A>G mutations, double mutation (mtDNA 4401A>G and 4435A>G) as well as wild type were used as templates. Specifically amplified mtDNA 234 bp fragments between 4344 - 4577 using nested PCR and digested the PCR purified products simultaneously with two restriction enzymes BfaI and NlaIII. The products were identified by polyacrylamide gel electrophoresis.
Results: Electrophoresis results showed that electrophoresis bands were specific among different samples.
Conclusions: This study established a convenient, accurate, and suitable for clinical determination of mtDNA 4401 A>G and 4435A>G mutations associated with essential hypertension testing using the new method.

• 出版日期2015
• 单位温州医科大学