Direct analysis of dried blood spots coupled with mass spectrometry: concepts and biomedical applications

作者：Deglon Julien; Thomas Aurelien; Mangin Patrice; Staub Christian^*

来源：Analytical and Bioanalytical Chemistry, 2012, 402(8): 2485-2498.

DOI：10.1007/s00216-011-5161-6

• Dried blood spots
• Direct LC-MS-MS analysis
• Ambient MS
• Automated on-line DBS

Because of the emergence of dried blood spots (DBS) as an attractive alternative to conventional venous plasma sampling in many pharmaceutical companies and clinical laboratories, different analytical approaches have been developed to enable automated handling of DBS samples without any pretreatment. Associated with selective and sensitive MS-MS detection, these procedures give good results in the rapid identification and quantification of drugs (generally less than 3 min total run time), which is desirable because of the high throughput requirements of analytical laboratories. The objective of this review is to describe the analytical concepts of current direct DBS techniques and to present their advantages and disadvantages, with particular focus on automation capacity and commercial availability. Finally, an overview of the different biomedical applications in which these concepts could be of major interest will be presented.

• 出版日期2012-3