An liquid chromatography-tandem mass spectrometry assay for determination of trace amount of new antifungal drug iodiconazole in human plasma

作者：Gao, Shouhong; Tao, Xia; Sun, Lianna; Sheng, Chunquan; Zhang, Wannian; Yun, Yunlei; Li, Jingxian; Miao, Haijun^*; Chen, Wansheng

来源：Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, 2009, 877(4): 382-386.

DOI：10.1016/j.jchromb.2008.12.034

• Iodiconazole
• LC-MS/MS
• Antifungal drug
• Human plasma

A simple, rapid and sensitive LC-MS/MS method for determination of trace amount of new antifungal drug iodiconazole in human plasma was developed (1-(1H-1,2,4-triazole)-2-(2,4-diflurophenyl)-3-[N-methyl-N-(3-chlor-benzyl)amino]-2-propanol), was used as internal standard (IS). The analytes were extracted by liquid-liquid extraction with n-hexane after internal standard spiked. The separation was performed by a ZORBAX SB-C-18 column (3.5 mu m, 2.1 mm x 100 mm) with an isocratic mobile phase consisting of methanol-water-formic acid (50:50:0.05, v/v/v) at a flow rate of 0.3 mL/min. The lower limit of quantification (LLOQ) was 0.10 ng/mL. This method was successfully used to determine the concentration of iodiconazole in human plasma following dermal administration.

• 出版日期2009-2-1
• 单位天津市中医药研究院附属医院; 中国人民解放军第二军医大学