摘要
The properties of the screened mutants for hyper-production of citric acid induced by carbon (C-12(6+)) ion beams and X-ray irradiation were investigated in our current study. Among these mutants, mutant H4002 screened from C-12(6+) ion irradiation had a higher yield of citric acid production than the parental strain in a 250-ml shaking flash. These expanded submerged experiments in a bioreactor were also carried out for mutant H4002. The results showed that (177.7-196.0) g/L citric acid was accumulated by H4002 through exploiting corn meal hydrolysate (containing initial 200.0-235.7 g/L sugar) with the productivity of (2.96-3.27) g/(L.h). This was especially true when the initial sugar concentration was 210 g/L, and the best economical citric acid production reached (187.5+/-0.7) g/L with a productivity of 3.13 g/(L.h). It was observed that mutant H4002 can utilize low-cost corn meal as a feedstock to efficiently produce citric acid. These results imply that the H4002 strain has the industrial production potentiality for citric acid and offers strong competition for the citric acid industry.
- 出版日期2014-11
- 单位中国科学院大学; 中国科学院近代物理研究所