Differential IFN-gamma production by adult and neonatal blood CD56(+) natural killer (NK) and NK-like-T cells in response to Trypanosoma cruzi and IL-15

作者：Guilmot A; Carlier Y; Truyens C^*

来源：Parasite Immunology, 2014, 36(1): 43-52.

DOI：10.1111/pim.12077

• human neonate and adult
• innate immunity
• Interferon-gamma
• natural killer cell
• T cell
• Trypanosoma cruzi

Early interferon-gamma (IFN-) release by innate cells is critical to direct type 1 immune response able to control intracellular pathogens like Trypanosoma cruzi. Although CD56(bright) natural killer (NK) cells are reported to be potent early IFN- producers, other CD56(+) cells like CD56(dim) NK cells and NK-like T cells have recently been shown to also release IFN-. We have here studied the contribution of each CD56(+) lymphocyte populations in early IFN- production in both adults and neonates. On this purpose, we analysed the kinetics of IFN- production by RT-PCR, ELISA and flow cytometry from 2h onwards after T.cruzi and IL-15 stimulation and sought for the responding CD56(+) cells. CD56(bright) and CD56(dim)CD16(-) NK cells were the more potent IFN- early producers in response to IL-15 and parasites in adults and neonates. In both age groups, the majority of IFN- producing cells were NK cells. However, on the contrary to neonates, CD3(+)CD56(+) NK-like T cells and CD3(+)CD56(-) classical' T cells also contributed to early IFN- production in adults. Altogether, our results support that whereas NK cells responded almost similarly in neonates and adults, cord blood innate CD56(+) and CD56(-) T cells displayed major quantitative and qualitative defects that could contribute to the well-known neonatal immune immaturity.

• 出版日期2014-1