Age-associated de-repression of retrotransposons in the Drosophila fat body, its potential cause and consequence

作者：Chen, Haiyang^*; Zheng, Xiaobin; Xiao, Danqing; Zheng, Yixian^*

来源：AGING CELL, 2016, 15(3): 542-552.

DOI：10.1111/acel.12465

• aging
• DNA damage
• heterochromatin
• Lamin-B
• lamin Dm0
• retrotransposons

Eukaryotic genomes contain transposable elements (TE) that can move into new locations upon activation. Since uncontrolled transposition of TEs, including the retrotransposons and DNA transposons, can lead to DNA breaks and genomic instability, multiple mechanisms, including heterochromatin-mediated repression, have evolved to repress TE activation. Studies in model organisms have shown that TEs become activated upon aging as a result of age-associated deregulation of heterochromatin. Considering that different organisms or cell types may undergo distinct heterochromatin changes upon aging, it is important to identify pathways that lead to TE activation in specific tissues and cell types. Through deep sequencing of isolated RNAs, we report an increased expression of many retrotransposons in the old Drosophila fat body, an organ equivalent to the mammalian liver and adipose tissue. This de-repression correlates with an increased number of DNA damage foci and decreased level of Drosophila lamin-B in the old fat body cells. Depletion of the Drosophila lamin-B in the young or larval fat body results in a reduction of heterochromatin and a corresponding increase in retrotransposon expression and DNA damage. Further manipulations of lamin-B and retrotransposon expression suggest a role of the nuclear lamina in maintaining the genome integrity of the Drosophila fat body by repressing retrotransposons.

• 出版日期2016-6
• 单位有害生物控制与资源利用国家重点实验室; 中山大学