科研之友
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摘要
A sensitive and selective liquid chromatography mass spectrometry (LC-MS) method for determination of isocorynoxeine in rat plasma was developed. After addition of midazolam as internal standard (IS), sample preparation was performed by using liquid-liquid extraction with ethyl acetate. Chromatographic separation was achieved on a Zorbax SB-C18 (2.1mmx150mm, 5 mu m) column with acetonitrile-0.1% formic acid as mobile phase with gradient elution. Electrospray ionization (ESI) source was applied and operated in positive ion mode; selective ion monitoring (SIM) mode was used to quantify using target fragment ions m/z 383 for isocorynoxeine and m/z 326 for the IS. Calibration plots were linear over the range of 5-500ng/mL for isocorynoxeine in plasma. Lower limit of quantification (LLOQ) for isocorynoxeine was 5ng/mL. Mean recovery of isocorynoxeine from plasma was in the range of 93.6-96.6%. CV of intra-day and inter-day precision were both less than 15%. The newly developed method is simple, sensitive, and could function effectively in pharmacokinetic characterization of isocorynoxeine in rat plasma.
- 出版日期2013-6-1
- 单位温州医科大学
