An Alternative Method for Irones Quantification in Iris Rhizomes using Headspace Solid-phase Microextraction

作者：Roger B; Fernandez X^*; Jeannot V; Chahboun J

来源：Phytochemical Analysis, 2010, 21(5): 483-488.

DOI：10.1002/pca.1223

• HS-SPME-GC
• irones quantification
• iris rhizomes
• Iris germanica
• matrix effect

Introduction - The essential oil obtained from iris rhizomes is one of the most precious raw materials for the perfume industry. Its fragrance is due to irones that are gradually formed by oxidative degradation of iridals during rhizome ageing.
Objective - The development of an alternative method allowing irone quantification in iris rhizomes using HS-SPME-GC.
Methodology - The development of the method using HS-SPME-GC was achieved using the results obtained from a conventional method, i.e. a solid-liquid extraction (SLE) followed by irone quantification by CG.
Results - Among several calibration methods tested, internal calibration gave the best results and was the least sensitive to the matrix effect. The proposed method using HS-SPME-GC is as accurate and reproducible as the conventional one using SLE. These two methods were used to monitor and compare irone concentrations in iris rhizomes that had been stored for 6 months to 9 years.
Conclusion - Irone quantification in iris rhizome can be achieved using HS-SPME-GC. This method can thus be used for the quality control of the iris rhizomes. It offers the advantage of combining extraction and analysis with an automated device and thus allows a large number of rhizome batches to be analysed and compared in a limited amount of time.

• 出版日期2010-10