A Novel Botulinum Neurotoxin, Previously Reported as Serotype H, Has a Hybrid-Like Structure With Regions of Similarity to the Structures of Serotypes A and F and Is Neutralized With Serotype A Antitoxin

作者：Maslanka Susan E^*; Luquez Carolina; Dykes Janet K; Tepp William H; Pier Christina L; Pellett Sabine; Raphael Brian H; Kalb Suzanne R; Barr John R; Rao Agam; Johnson Eric A

来源：Journal of Infectious Diseases, 2016, 213(3): 379-385.

DOI：10.1093/infdis/jiv327

• botulinum toxin
• Clostridium botulinum
• serotype
• botulism
• neutralization
• neuronal cell-based assay

Botulism is a potentially fatal paralytic disease caused by the action of botulinum neurotoxin (BoNT) on nerve cells. There are 7 known serotypes (A-G) of BoNT and up to 40 genetic variants. Clostridium botulinum strain IBCA10-7060 was recently reported to produce BoNT serotype B (BoNT/B) and a novel BoNT, designated as BoNT/H. The BoNT gene (bont) sequence of BoNT/H was compared to known bont sequences. Genetic analysis suggested that BoNT/H has a hybrid-like structure containing regions of similarity to the structures of BoNT/A1 and BoNT/F5. This novel BoNT was serologically characterized by the mouse neutralization assay and a neuronal cell-based assay. The toxic effects of this hybrid-like BoNT were completely eliminated by existing serotype A antitoxins, including those contained in multivalent therapeutic antitoxin products that are the mainstay of human botulism treatment.

• 出版日期2016-2-1