科研之友
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摘要
Very high casein content and good coagulation properties previously observed in some Ethiopian goat breeds led to investigating the alpha(s1)-casein (CSN1S1) gene in these breeds. Selected regions of the CSN1S1 gene were sequenced in 115 goats from 5 breeds (2 indigenous: Arsi-Bale and Somali, 1 exotic: Boer, and 2 crossbreeds: Boer x Arsi-Bale and Boer x Somali). The DNA analysis resulted in 35 new mutations: 3 in exons, 3 in the 5%26apos; untranslated region (UTR), and 29 in the introns. The mutations in exons that resulted in an amino acid shift were then picked to evaluate their influence on individual casein content (alpha(s1)-, alpha(s2)-, beta-, and kappa-CN), micellar size, and coagulation properties in the milk from the 5 goat breeds. A mutation at nucleotide 10657 (exon 10) involved a transversion: CAG -%26gt; CCG, resulting in an amino acid exchange Gln(77)-%26gt; Pro(77). This mutation was associated with the indigenous breeds only. Two new mutations, at nucleotide 6072 (exon 4) and 12165 (exon 12), revealed synonymous transitions: GTC -%26gt; GTT in Val(15) and AGA -%26gt; AGG in Arg(100) of the mature protein. Transitions G -%26gt; A and C -%26gt; T at nucleotides 1374 and 1866, respectively, occurred in the 5%26apos; UTR, whereas the third mutation involved a transversion T -%26gt; G at nucleotide location 1592. The goats were grouped into homozygote new (CC), homozygote reference (AA), and heterozygote (CA) based on the nucleotide that involved the transversion. The content of alpha(s1)-CN (15.32 g/kg) in milk samples of goats homozygous (CC) for this newly identified mutation, Gln(77)-%26gt; Pro(77) was significantly higher than in milks of heterozygous (CA; 9.05 g/kg) and reference (AA; 7.61 g/kg) genotype animals. The alpha(s2)-, beta-, and kappa-CN contents showed a similar pattern. Milk from goats with a homozygous new mutation had significantly lower micellar size. Milk from both homozygote and heterozygote new-mutation goats had significantly shorter coagulation rate and stronger gel than the reference genotype. Except the transversion, the sequence corresponded to allele A and presumably derived from it. Therefore, this allele is denoted by A(3). All goats from the reference genotype (AA) were homozygous for the allele at nucleotide position 1374 and 1866, whereas all mutations in the 5%26apos; UTR existed in a heterozygous form in both heterozygous (CA) and the new mutation (CC) genotype. The newly identified mutation (CC) detected in some of the goat breeds is, therefore, important in selection for genetic improvement and high-quality milk for the emerging goat cheese-producing industries. The finding will also benefit farmers raising these goat breeds due to the increased selling price of goats. Further studies should. investigate the effect of this amino acid exchange on the secondary and tertiary structure of the alpha(s1)-CN molecule and on the susceptibility of peptide hydrolysis by digestive enzymes.
- 出版日期2013-8
