Detection of two Bartonella tamiae-like sequences in Amblyomma americanum (Acari : Ixodidae) using 16S-23S intergenic spacer region-specific primers

作者：Billeter Sarah A; Miller Melissa K; Breitschwerdt Edward B; Levy Michael G^*

来源：Journal of Medical Entomology, 2008, 45(1): 176-179.

DOI：10.1603/0022-2585(2008)45[176:DOTBTS]2.0.CO;2

• Bartonella
• Amblyomma americanum
• PCR

Four hundred and sixty-six questing Amblyomma americanum (L.) (Acari: Ixodidae) from Carolina County, VA, and 98 questing A. americanum from Chatham County, NC, were screened by polymerase chain reaction (PCR) for the Bartonella 16S-23S intergenic spacer region. Two amplicons, approximate to 270-280 bp, were detected in two ticks from Virginia. Based upon PCR and sequencing, an adult male and adult female tick harbored DNA sequences closely related to Bartonella tamiae (DQ395180). Bartonella DNA was not detected in A. americanum from North Carolina. Potential transmission of Bartonella spp. by A. arnericanuin should be the focus of future experimental studies.

• 出版日期2008-1