Anti-tumor Effects of a Recombinant Fowlpox Virus Expressing Apoptin on Human Cervical Carcinoma in Vivo and in Vitro

作者：Zhu Ji hong; Li Xiao; Sun Li li; Zhang Mu chun; Kan Shi fu; Liu Lei; Huang Hai yan; Yang Guo hua; Piao Bing guo; Jin Ning yi^*

来源：Chemical Research in Chinese Universities, 2011, 27(4): 646-650.

• Apoptin gene
• Chicken anemia virus
• Human cervical carcinoma
• Anti-tumor effect

Apoptin is a chicken anemia virus-derived, p53-independent, bcl-2-insensitive apoptotic protein with the ability to specifically induce apoptosis of various human tumor cells, but not of normal diploid cells. To explore the application of apoptin in tumor gene therapy, we used a recombinant fowlpox virus expressing apoptin protein (vFV-Apoptin) to investigate the anti-tumor effectes of vFV-Apoptin on human cervical carcinoma(HeLa) cells in vivo and in vitro through 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide(MTT) assay, acridine orage/ethidium bromide(AO/EB) and annexin V staining test, respectively. The results show that vFV-Apoptin inhibites the proliferation of HeLa cells in vitro through inducing the apoptosis of HeLa cells, and the inhibition effect of vFV-Apoptin has a dose-effect and time-effect relationship. The results of animal models show that vFV-Apoptin significantly inhibits tumor growth, extends the lifespan of animals and improves the mean survival. Experimental results indicate that vFV-Apoptin has a potential application in the tumor gene therapy.

• 出版日期2011-7
• 单位吉林大学; 中国人民解放军军事医学科学院; 吉林省肿瘤医院