A novel amperometric biosensor based on ZnO : Co nanoclusters for biosensing glucose

作者：Zhao Z W^*; Chen X J; Tay B K; Chen J S; Han Z J; Khor K A

来源：Biosensors and Bioelectronics, 2007, 23(1): 135-139.

DOI：10.1016/j.bios.2007.03.014

• ZnO
• nanoclusters
• glucose biosensor
• cross-linking

ZnO:Co nanoclusters were synthesized by nanocluster-bearn deposition with averaged particle size of 5 nm and porous structure, which were for the first time adopted to construct a novel amperometric glucose biosensor. Glucose oxidase was immobilized into the ZnO:Co nanocluster-assembled thin film through Nafion-assisted cross-linking technique. Due to the high specific active sites and high electrocatalytic activity of the ZnO:Co nanoclusters, the constructed glucose biosensor showed a high sensitivity of 13.3 mu A/mA cm(2). The low detection limit was estimated to be 20 mu M (S/N = 3) and the apparent Michaelis-Menten constant was found to be 21 mM, indicating the high affinity of the enzyme on ZnO:Co nanoclusters to glucose. The results show that the ZnO:Co nanocluster-assembled thin films with nanoporous structure and nanocrystallites have potential applications as platforms to immobilize enzyme in biosensors.

• 出版日期2007-8-30
• 单位东南大学; 南阳理工学院