Simultaneous Determination of Amiloride and Hydrochlorothiazide in a Compound Tablet by Diffuse Reflectance Spectroscopy and Chemometrics

作者：Tang, J.; Li, X.; Feng, Y.; Liang, B.^*

来源：Journal of Applied Spectroscopy, 2016, 83(4): 710-716.

DOI：10.1007/s10812-016-0352-8

• compound amiloride hydrochloride tablet
• partial least squares
• support vector regression
• ultraviolet-visible-shortwave near-infrared diffuse reflectance spectroscopy

This paper studies the simultaneous determination of amiloride hydrochloride (AMH) and hydrochlorothiazide (HCTZ) in amiloride hydrochloride tablets by ultraviolet-visible-shortwave near-infrared diffuse reflectance spectroscopy (UV-Vis-swNIR DRS) and chemometrics. Quantitative models for the two components were established by partial least squares (PLS) and support vector regression (SVR), respectively. For the PLS models of AMH and HCTZ, the determination coefficient R-2 of the calibration set was 0.9503 and 0.9538, and the coefficient R-2 of the prediction set was 0.8983 and 0.9260, respectively. The root mean square error of the calibration set (RMSEC) was 0.8 mg and 8.1 mg, while the root mean square error of the prediction set (RMSEP) was 1.0 mg and 8.7 mg, respectively. For the SVR models of AMH and HCTZ, the R-2 of the calibration set was 0.9668 and 0.9609; the R-2 of the prediction set was 0.9145 and 0.9446, respectively. The RMSEC was 0.7 and 7.5 mg, and the RMSEP was 0.9 and 8.9 mg, respectively. The results show that SVR modeling has a satisfactory prediction effect. The proposed method based on UV-vis-swNIR and chemometrics is efficient, nondestructive, and expected to be used for online quality monitoring in the production of drugs.

• 出版日期2016-9
• 单位四川大学