Detection of smell print differences between nonmalignant and malignant prostate cells with an electronic nose

作者：Roine Antti^*; Tolvanen Mikko; Sipilainen Miki; Kumpulainen Pekka; Helenius Merja A; Lehtimaki Terho; Vepsalainen Jouko; Keinanen Tuomo A; Hakkinen Merja R; Koskimaki Juha; Veskimae Erik; Tuokko Antti; Visakorpi Tapio; Tammela Teuvo L; Sioris Thanos; Paavonen Timo; Lekkala Jukka; Helle Hannu; Oksala Niku K J

来源：Future Oncology, 2012, 8(9): 1157-1165.

DOI：10.2217/FON.12.93

• cell lines
• electronic nose
• machine olfaction
• olfactory detection
• prostate cancer
• volatile organic compounds

Aim: To determine whether an electronic nose can differentiate cultured nonmalignant and malignant prostatic cells from each other and whether the smell print is secreted to the surrounding medium. Materials %26 methods: Prostatic nonmalignant (EP-156T and controls) and malignant (LNCaP) cell lines, as well as conditioned and unconditioned media, were collected. The smell prints of the samples were analyzed by a ChemPro (R) 100 electronic nose device. The data were normalized and dimension reduction was conducted. The samples were classified and misclassification rates were calculated. Results: The electronic nose differentiated the nonmalignant and malignant cell lines from each other, achieving misclassification rates of 2.9-3.6%. Cells did not differ from the conditioned medium but differed from the unconditioned medium (misclassification rates: 0.0-25.6%). Conclusion: Malignant and nonmalignant prostatic cell lines have distinct smell prints. Prostatic cancer cells seem to modify the smell print of their medium.

• 出版日期2012-9