科研之友
微信
新浪微博
Facebook
分享链接
摘要
aSoybean (Glyeine max (L.) Merr.) oil typically contains 8% alpha-linolenic acid that is highly unstable and easily oxidized. This property is undesirable in many food and industrial applications. Genetic strategies for reducing alpha-linolenic acid content would enhance the commercial value. However, genetic resources for low alpha-linolenic acid content are limited among natural soybean variations. Microsomal omega-3-fatty acid desaturase (FAD3) is responsible for the synthesis of alpha-linolenic acid in the polyunsaturated fatty acid pathway. There are four FAD3 homologs (Glyma02g39230,Glymallg27190, Glyma14g37350 and Glymal8g06950) in the soybean genome. While non-functional alleles have been reported for Glyma02g39230 (GmE4D3-1a) and Glymal4g37350 (GmFAD3-1b), little variation is seen in Glymal8g06.9.50 (GmFAD3-2a). We isolated seven mutant GmFAD3-2a alleles, each containing a single-nucleotide substitution, from 39,100 independent mutant lines by using targeting induced local lesions in genomes (TILLING). Analysis of GmFAD3-2a transcripts and enzyme activities revealed that one missense mutant, %26apos;Pl-A9%26apos;, contains a non-functional allele of GmE4D3-2a. By combining three non-functional alleles (GmFAD3-1a, GmFAD3-1b, and GmFAD3-2a), we generated soybean lines containing %26lt;2% alpha-linolenic acid in their seeds. The reverse-genetics-based development of novel mutant alleles in the fatty acid metabolic pathway will allow the improvement of soybean with better oil quality through conventional breeding.
- 出版日期2014-12
