科研之友
微信
新浪微博
Facebook
分享链接
摘要
The complete genome of Populus trichocarpa has been available for years, however, relatively little effort has been made to map and quantify the transcriptome for this important model organism. Here, we applied Illumina sequencing to systematically investigate the leaf transcriptomes derived from P. trichocarpa seedlings grown in normal condition and drought stress. On the basis of the available Populus genome, we defined gene structures and identified a number of novel transcripts and upstream open reading frames, which may be informative for understanding drought adaption of woody plants. We obtained 33,044 genes expressed in leaves covering similar to 80 % of the available P. trichocarpa gene models and 5689 genes were differentially expressed during drought stress. About 38.9 % of the expressed genes show alternative splicing (AS) patterns. Interestingly, the number of AS events and the expression of some AS genes increased when the plants were subjected to drought. At the physiological level, photosynthetic rates, stomatal conductance, and leaf water potential were significantly reduced after water stress. This was accompanied by strong transcriptional remodeling of energy metabolism, cell growth, carbohydrate metabolism, and cellular homeostasis in P. trichocarpa. In addition, genes involved in photosynthesis, cell wall organization, and osmoprotectants metabolism that may specially modulate the drought stress responses of P. trichocarpa are highlighted. Our analysis provides insight into the transcriptional responses of P. trichocarpa to drought stress and is expected to serve as valuable transcriptome resources for woody plants.
- 出版日期2015-6
- 单位北京林业大学
