A new assay format for NF-kappa B based on a DNA triple helix and a fluorescence resonance energy transfer

作者：Altevogt Dominik; Hrenn Andrea; Kern Claudia; Clima Lilia; Bannwarth Willi; Merfort Irmgard^*

来源：Organic and Biomolecular Chemistry, 2009, 7(19): 3934-3939.

DOI：10.1039/b906447h

Herein we report a feasibility study for a new concept to detect DNA binding protein NF-kappa B based on a DNA triple helix formation in combination with a fluorescence resonance energy transfer (FRET). The new principle avoids expensive antibodies and radioactivity and might have implications for assays of other DNA binding proteins.

• 出版日期2009