Botanical pesticides and their human health safety on the example of Citrus sinensis essential oil and Oulema melanopus under laboratory conditions

作者：Zarubova Lenka; Kourimska Lenka^*; Zouhar Miloslav; Novy Pavel; Douda Ondrej; Skuhrovec Jiri

来源：Acta Agriculturae Scandinavica Section B-Soil and Plant Science, 2015, 65(1): 89-93.

DOI：10.1080/09064710.2014.959556

• crop protection
• biopesticides
• d-limonene
• larvicidal
• organic agriculture
• Triticum aestivum

Biopesticides derived from plants have recently received increasing interest as an alternative to conventional chemicals suitable for integrated pest management and organic farming. In this study, Citrus sinensis (Osbeck) essential oil was tested for its potential for wheat protection against Oulema melanopus (L.). The chemical composition of the essential oil was analyzed by gas chromatography-mass spectrometry (GC-MS). Twenty-four compounds were identified in the oil among which d-limonene was a predominant component, followed by myrcene, alpha-pinene, linalool, sabinene, decanal, and other minor components. Direct contact toxicity assay showed the effectiveness of the essential oil against O. melanopus larvae causing the mortality of 85% during 48 hours. The persistence study revealed high dissipation rate of the oil from treated plants, whereas concentrations lower than 0.01 g/kg were detected by GC-MS 5 min after the application of the oil, suggesting its environmental and food safety. These results, together with previous literature reports reviewed in this study, indicate the great potential of the C. sinensis essential oil for future use in crop protection against insect pests.

• 出版日期2015-1-2