A substrate for the detection of broad specificity alpha-L-arabinofuranosidases with indirect release of a chromogenic group

作者：Borsenberger Vinciane; Dornez Emmie; Desrousseaux Marie Laure; Courtin Christophe M; O'Donohue Michael J; Faure Regis^*

来源：Tetrahedron Letters, 2013, 54(24): 3063-3066.

DOI：10.1016/j.tetlet.2013.03.136

• Carbohydrate
• 4-Nitrocatechol
• AXH-d(3)
• alpha-L-Arabinofuranosidase

The synthesis of a compound containing a 4-nitrocatechol bound to two vicinal alpha-L-arabinofuranosyl moieties through a linker arm was achieved using a sulfate protecting group to facilitate selective alkylation of one aromatic hydroxyl. Several alpha-L-arabinofuranosidases displaying different selectivities were tested and a simple microtiter plate-based assay was developed. The observed resistance of the compound to alpha-L-arabinofuranosidase-mediated hydrolysis makes it suitable for the identification of enzymes that are able to accommodate bis-arabinofuranosylated moieties.

• 出版日期2013-6-12