Dual-Modality Positron Emission Tomography/Optical Image-Guided Photodynamic Cancer Therapy with Chlorin e6-Containing Nanomicelles

作者：Cheng, Liang^*; Kamkaew, Anyanee; Sun, Haiyan; Jiang, Dawei; Valdovinos, Hector F.; Gong, Hua; England, Christopher G.; Goel, Shreya; Barnhart, Todd E.; Cai, Weibo^*

来源：ACS Nano, 2016, 10(8): 7721-7730.

DOI：10.1021/acsnano.6b03074

• PEG-Ce 6 nanomicelles
• PET imaging
• fluorescence imaging
• EPR effect
• photodynamic therapy

Multifunctional nanoparticles with combined diagnostic and therapeutic functions show great promise in nanomedicine. Herein, we develop an organic photo dynamic therapy (PDT) system based on polyethylene glycol (PEG)-coated nanomicelles conjugated with similar to 20% chlorin e6 (PEG-Ce 6 nanomicelles), which functions as an optical imaging agent, as well as a PDT agent. The formed PEG-Ce 6 nanomicelles with the size of similar to 20 nm were highly stable in various physiological solutions for a long time. Moreover, Ce 6 can also be a Cu-64 chelating agent for in vivo positron emission tomography (PET). By simply mixing, more than 90% of Cu-64 was chelator-free labeled on PEG-Ce 6 nanomicelles, and they also showed high stability in serum conditions. Both fluorescence imaging and PET imaging revealed that PEG-Ce 6 nanomicelles displayed high tumor uptake (13.7 +/- 2.2%ID/g) after intravenous injection into tumor-bearing mice at the 48 h time point. In addition, PEG-Ce 6 nanomicelles exhibited excellent PDT properties upon laser irradiation, confirming the theranostic properties of PEG-Ce 6 nanomicelles for imaging and treatment of cancer. In addition, PDT was not shown to render any appreciable toxicity. This work presents a theranostic platform based on polymer nanomicelles with great potential in multimodality imaging guided photodynamic cancer therapy.

• 出版日期2016-8
• 单位苏州大学