A Glycoprotein Extracted from Golden Oyster Mushroom Pleurotus citrinopileatus Exhibiting Growth Inhibitory Effect against U937 Leukemia Cells

作者：Chen Jian Nan; Wang Yuh Tai; Wu James Swi Bea^*

来源：Journal of Agricultural and Food Chemistry, 2009, 57(15): 6706-6711.

DOI：10.1021/jf901284s

• Mushroom
• leukemic
• antitumor
• glycoprotein

Mushrooms have become popular sources of natural antitumor, antiviral, antibacterial, antioxidative, and immunomodulatory agents. Golden oyster mushroom, Pleurotus citrinopileatus, is a common mushroom in oriental countries for human consumption. We isolated a functional protein (PCP-3A) from the fresh fruiting body of this mushroom. The isolation procedure included ammonium sulfate fractionation, DEAE-Sepharose CL-6B ion exchange chromatography, and Sephacryl S-300 gel filtration. Electrophoresis demonstrated that PCP-3A is a glycoprotein composed of 10 subunits, each approximately 45.0 kDa in size. In vitro cell study showed that PCP-3A at a concentration about 12.5 mu g/mL inhibits the proliferation of human tumor cell line U937, in a time- dependent manner (24, 48, and 72 h). It failed to agglutinate rabbit and human erythrocytes, excluding its possibility from being a lectin. Flow cytometry revealed that it is capable of inhibiting the growth of U937 cells by way of S phase arrest and apoptotic induction. We suggest that PCP-3A is worth further investigating for antitumor use.

• 出版日期2009-8-12