A novel Rieske-type protein derived from an apoptosis-inducing factor-like (AIFL) transcript with a retained intron 4 induces change in mitochondrial morphology and growth arrest

作者：Murata Yasuhiko; Furuyama Isao; Oda Shoji; Mitani Hiroshi^*

来源：Biochemical and Biophysical Research Communications, 2011, 407(1): 92-97.

DOI：10.1016/j.bbrc.2011.02.115

• AIFL
• Rieske domain
• Pyr_redox domain
• [2Fe-2S] cluster
• Intron retention

Apoptosis-inducing factor-like (AIFL) protein contains a Rieske domain and pyridine nucleotide-disulfide oxidoreductase (Pyr_redox) domain that shows 35% homology to that of apoptosis-inducing factor (AlF) protein. We identified a novel major transcript of the medaka (Oryzias latipes) AIFL gene that retained intron 4 (AIFL-14) in embryos and tissues from adult fish. The product of this transcript, AIFL-I4 protein, lacked the Pyr_redox domain because of a nonsense codon in intron 4. Both AIFL-I4 and full-length AIFL (fAIFL) transcripts were highly expressed in the brain and late embryos, and relative fAIFL and AIFL-I4 expression levels differed among tissues. Transient expression of AIFL-I4 and fAIFL tagged with GFP showed that AIFL-I4 localized in the nucleus, while fAIFL localized throughout the cytoplasm. We also found that overexpression of AIFL-14 induced a change in mitochondrial morphology and suppression of cell proliferation. AIFL-I4 mutant with a lesion in [2Fe-2S] cluster binding site of the Rieske domain did not induce these phenotypes. This report is the first to demonstrate nuclear localization of a Rieske-type protein translated from the AIFL gene. Our data suggested that the [2Fe-2S] cluster binding site was essential for the nuclear localization and involved in mitochondrial morphology and suppression of cell proliferation.

• 出版日期2011-4-1