Stability of Staphylococcus aureus Phage ISP after Freeze-Drying (Lyophilization)

作者:Merabishvili Maia*; Vervaet Chris; Pirnay Jean Paul; De Vos Daniel; Verbeken Gilbert; Mast Jan; Chanishvili Nino; Vaneechoutte Mario
来源:PLos One, 2013, 8(7): e68797.
DOI:10.1371/journal.pone.0068797

摘要

Staphylococcus aureus phage ISP was lyophilized, using an Amsco-Finn Aqua GT4 freeze dryer, in the presence of six different stabilizers at different concentrations. Stability of the lyophilized phage at 4 degrees C was monitored up to 37 months and compared to stability in Luria Bertani broth and physiological saline at 4 degrees C. Sucrose and trehalose were shown to be the best stabilizing additives, causing a decrease of only 1 log immediately after the lyophilization procedure and showing high stability during a 27 month storage period.

  • 出版日期2013-7-2