Acetylation of aromatic cysteine conjugates by recombinant human N-acetyltransferase 8

作者：Deol Reema; Josephy P David^*

来源：Xenobiotica, 2017, 47(3): 202-207.

DOI：10.1080/00498254.2016.1178410

• Benzylcysteine
• 4-nitrobenzylcysteine
• mercapturic acid pathway
• NAT8
• N-acetyltransferase
• 1-menaphthylcysteine

1. The mercapturic acid (MA) pathway is a metabolic route for the processing of glutathione conjugates to MA (N-acetylcysteine conjugates). An N-acetyltransferase enzyme, NAT8, catalyzes the transfer of an acetyl group from acetyl-CoA to the cysteine amino group, producing a MA, which is excreted in the urine. We expressed human NAT8 in HEK293T cells and developed an HPLC-MS method for the quantitation of the S-aryl-substituted cysteine conjugates and their MA.
2. We measured the activity of the enzyme for acetylation of benzyl-, 4-nitrobenzyl-, and 1-menaphthylcysteine substrates.
3. NAT8 catalyzed the acetylation of all three cysteine conjugates with similar MichaelisMenten kinetics.

• 出版日期2017