A Rapid Way to Map Outheld-Wing Mutation in Drosophila melanogaster

作者：Yu Bo; Cao Huojun; Zhao Chunjiang; Wu Changxin; Deng Xuemei^*

来源：Asian Journal of Animal and Veterinary Advances, 2011, 6(3): 242-254.

DOI：10.3923/ajava.2011.242.254

• Spontaneous mutation
• Beijing spreading wing
• P element mapping
• SNP mapping
• twist

Map-based positional cloning of Drosophila melanogaster genes is hampered by both the time-consuming, labor-intensive, error-prone nature of traditional methods for genetic mapping-SNP mapping is costly and P element mapping is low-resolution for limit number of P elements. Here we used two strategies to locate a spontaneous mutant screened in our lab. One is P element mapping to map the mutant in a 33 k-interval on the right arm of the second chromosome; another is combination of P element mapping and a Single-Nucleotide Polymorphism (SNP) mapping together to map the mutant in a very small interval, about 5 kb, on the right arm of the second chromosome efficiently. At last, we guessed that the mutant phenotype maybe resulted from the overexpression of twist gene. Comparing the two strategies, the method combining the P insertion mapping and SNP mapping could ward off their drawbacks. Therefore, the strategy of combination of the two methods provides a rapid protocol for mapping mutations.

• 出版日期2011-3
• 单位中国农业大学