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摘要
The detection of pidotimod in plasma is frequently troublesome due mainly to the endogenous interference caused by some analogues in plasma. Moreover, the application of conventional reversed phase columns in high-performance liquid chromatography (HPLC) for the detection has not been reported to date. In this study, a low-cost and effective reversed phase HPLC method using an ultraviolet detector at 210 nm was developed, by systematically optimizing protein precipitation agents, the polarity and the pH value of the mobile phase. Pidotimod was successfully separated from the endogenous interference in plasma using equal-volume 10 % perchloric acid as protein precipitation agent, and 0.01 M sodium dihydrogen phosphate (pH 4.0 with concentrated phosphoric acid)-methanol-isopropanol (97:2:1, v/v) as mobile phase. After validation of specificity, linearity, recovery, precision, accuracy and stability, this method has been successfully applied to a pharmacokinetic study in rats.
- 出版日期2014
- 单位中国药科大学
