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摘要
In pH 4.0 succinic acidsodium hydroxide buffer solution, laccase catalyzed the oxidization of guaiacol substrate to form red particles, which exhibited a strong resonance scattering (RS) peak at 590 nm. Under the chosen conditions, as the laccase increased, the RS intensity (Delta I) increased linearly. The Delta I was proportional to laccase activity in the range of 0.10-1.2 U/mL, with a regression equation of Delta I = 734.0 U-laccase - 9.7, and a detection limit of 0.05 U/mL. This RS method was applied to the detection of laccase activity in real samples, and the results were agreement with those from spectrophotometry.
- 出版日期2011-12
- 单位广西师范大学
